Uji Aktivitas Ekstrak Kulit Mangga Gedong Gincu (Mangifera Indica L.) Terhadap Pertumbuhan Bakteri Propionibacterium Acnes ATCC 6919

Azfa Marsya Sholichah; Afifa Khairinnisa; Rama Samara Brajawikalpa

doi:10.31004/koloni.v5i3.1392

Authors

Azfa Marsya Sholichah Universitas Swadaya Gunung Jati
Afifa Khairinnisa Universitas Swadaya Gunung Jati
Rama Samara Brajawikalpa Universitas Swadaya Gunung Jati

DOI:

https://doi.org/10.31004/koloni.v5i3.1392

Keywords:

Mango Peel Extract, Propionibacterium Acnes, Antibacterial

Abstract

Propionibacterium acnes plays an important role in the pathogenesis of acne vulgaris, while increasing resistance to topical antibiotics has driven the development of natural antibacterial agents. Mango (Mangifera indica L.) peel of the Gedong Gincu variety contains bioactive compounds with potential antibacterial activity. Aim: To analyse the antibacterial activity of Gedong Gincu mango peel extract against the growth of Propionibacterium acnes ATCC 6919. Methods: A laboratory experimental study using a post-test only control group design was conducted. Samples were divided into six groups: positive control (clindamycin 1%), negative control (DMSO 10%), and treatment groups with extract concentrations of 25%, 50%, 75%, and 100%. Antibacterial activity was assessed using the well diffusion method, and data were analysed using the Shapiro-Wilk test, One-Way ANOVA, Least Significant Difference (LSD), and linear regression. Results: Gedong Gincu mango peel extract inhibited the growth of Propionibacterium acnes ATCC 6919, with mean inhibition zone diameters at concentrations of 25%, 50%, 75%, and 100% of 9.68 mm, 13.26 mm, 15.48 mm, and 18.01 mm, respectively. One-Way ANOVA showed a significant difference between groups (p<0.001), and linear regression showed a very strong positive relationship between extract concentration and inhibition zone diameter (R=0.991; R²=0.981). Conclusion: Gedong Gincu mango peel extract has antibacterial activity against Propionibacterium acnes ATCC 6919 that increases with extract concentration.

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