Formulasi dan Uji Antibakteri Sediaan Pasta Gigi Ekstrak Parijoto  (Medinilla speciosa) dengan Bovine Hidroksiapatit (BHA)

Teguh Imanto; Ronindya Gerry Aprastya

doi:10.31004/koloni.v5i2.893

Authors

Teguh Imanto Universitas Muhammadiyah Surakarta
Ronindya Gerry Aprastya Universitas Muhammadiyah Surakarta

DOI:

https://doi.org/10.31004/koloni.v5i2.893

Keywords:

antibacterial, bovine hydroxyapatite, dental caries, parijoto, toothpaste

Abstract

Dental caries is an oral health problem caused by bacteria such as Streptococcus mutans and Staphylococcus aureus. Parijoto fruit (Medinilla speciosa) contains secondary metabolites that have potential as antibacterial agent, while bovine hydroxyapatite (BHA) serves as a dental remineralizing agent. This study aims to formulate parijoto fruit extract toothpaste with BHA and evaluate its physical quality and antibacterial activity. This laboratory experimental study used four formulas, namely F0 (without extract), F1 (12.5% extract), F2 (15% extract), and F3 (17.5% parijoto extract). The extract was obtained by maceration method using 70% ethanol. Physical quality evaluation included organoleptic, homogeneity, pH, viscosity, and foam height, while antibacterial activity was tested by agar well diffusion method against Staphylococcus aureus and Streptococcus mutans. The Data were analyzed using statistical tests with significance level p < 0.05. Phytochemical screening results showed the presence of phenolics, flavonoids, saponins, and tannins. All formulas meet the physical quality requirements of toothpaste based on SNI 12-3524-1995. The pH values F0–F3 are 9,30 ± 0,05; 5,61 ± 0,03; 5,53 ± 0,03; and 5.75 ° 0.05. The variation of extract concentration significantly affected the viscosity and foam height (p < 0.05). Antibacterial test results showed that F3 has the highest inhibitory power against Staphylococcus aureus and significantly different from the positive control (p < 0.05). Based on the results of research, parijoto fruit extract with BHA has the potential as a toothpaste preparation with good physical quality and antibacterial activity.

References

Afni, N., Said, N., & Yuliet. (2015). Antibacterial Activity Test of Toothpaste of Betel Nut (Areca catechu L.) Extract Against Streptococcus mutans and Staphylococcus aureus. GALENIKA Journal of Pharmacy, 1(1), 48–58.

Anwar, A. I., Ruslin, M., Marlina, E., & Hasanuddin, H. (2025). Physicochemical analysis and application of sardinella fimbriata-derived hydroxyapatite in toothpaste formulations. BMC Oral Health, 25(1). https://doi.org/10.1186/s12903-025-05557-7

Chen, L., Al-Bayatee, S., Khurshid, Z., Shavandi, A., Brunton, P., & Ratnayake, J. (2021). Hydroxyapatite in oral care products—a review. Materials, 14(17). https://doi.org/10.3390/ma14174865

Eff, A. R. Y., Ramadhani, R. D., & Hurit, H. E. (2024). Formulasi Pasta Gigi Herbal Ekstrak Etanol Daun Rosemary (Rosmarinus officinalis Linn.). Archives Pharmacia, 6(1), 1–11. https://doi.org/10.47007/ap.v6i1.7462

El-Gar, Y. H. A., Etman, W. M., Genaid, T. M., & Al-Madboly, L. A. (2022). Potent Antibacterial and Antibiofilm Activities of a Synthetic Remineralizing Preparation of Nano-Hydroxyapatite Against Cariogenic Streptococcus mutans Using an Ex-vivo Animal Model. Frontiers in Dental Medicine, 3(March), 1–16. https://doi.org/10.3389/fdmed.2022.738326

Gintu, A. R., Kristian, E. B. E., & Martono, Y. (2020). Karakterisasi Pasta Gigi Berbahan Abrasif Hidroksiapatit (HAp). Jurnal Kimia Riset, 5(2), 120. https://doi.org/10.20473/jkr.v5i2.22503

Health research and development agency ministry of health of Indonesia. (2018). Laporan Riskesdas 2018 Nasional. Lembaga Penerbit Balitbangkes.

Imanto, T., Prasetiawan, R., & Wikantyasning, E. R. (2019). Formulasi dan Karakterisasi Sediaan Nanoemulgel Serbuk Lidah Buaya (Aloe Vera L.). Pharmacon: Jurnal Farmasi Indonesia, 16(1), 28–37. https://doi.org/10.23917/pharmacon.v16i1.8114

Ireland, I. N. (2018). Oral Health Oral Health. Retrieved May 5, 2026, from Pdfs.Semanticscholar.Org website: https://www.who.int/news-room/fact-sheets/detail/oral-health

Kresnawati, Y., & Mutmainah, M. (2023). FORMULASI DAN UJI STABILITAS FISIK SEDIAAN PASTA GIGI EKSTRAK BUAH DELIMA MERAH (Punica granatum L.). Parapemikir : Jurnal Ilmiah Farmasi, 12(3), 321. https://doi.org/10.30591/pjif.v12i3.5669

Kumalasari, M. L. F., & Andiarna, F. (2020). UJI FITOKIMIA EKSTRAK ETANOL DAUN KEMANGI (Ocimum basilicum L). Indonesian Journal for Health Sciences, 4(1), 39. https://doi.org/10.24269/ijhs.v4i1.2279

Kurniawan, F. L., Martiningtiyas, C. R., Yojana, R. M., JK, F. A., & Annisa, F. R. (2025). Pemanfaatan Limbah Kulit dan Daun Singkong sebagai Bahan Pasta Gigi Alami untuk Meningkatkan Kesehatan Gigi Masyarakat Desa Ginanjar, Sukabumi. Abdimas Galuh, 7(September), 1859–1866.

Luthfiana Nurulin Nafi’ah. (2022). REVIEW ARTICLE: AKTIVITAS FARMAKOLOGI TANAMAN PARIJOTO (Medinilla speciosa). Jurnal Riset Rumpun Ilmu Kesehatan, 1(1), 09–18. https://doi.org/10.55606/jurrikes.v1i1.172

Maduratna Setiawatie, E., Sandra Sari, D., Septa Wahyudadi, B., Fitria, E., Kurnia, S., Bargowo, L., & Apriliani Gani, M. (2021). Viability of Nigella sativa Toothpaste with SLS Compared Non-SLS on Fibroblast Cell Culture. Journal of International Dental and Medical Research, 14(2), 525–528. Retrieved from http://www.jidmr.com

Mintadoa, P. E., Yudistira, A., & Hariyanto, Y. A. (2024). Uji Aktivitas Antioksidan Ekstrak Etanol Lissoclinum patella yang Diperoleh dari Pantai Parentek Kabupaten Minahasa. Pharmacon, 13(2), 594–601. https://doi.org/10.35799/pha.13.2024.55990

Ningrum, W., Y, A. N., & Faizal, I. A. (2024). FORMULASI PASTA GIGI GEL EKSTRAK BUAH BELIMBING WULUH (Averrhoa bilimbi L.) SEBAGAI ANTIBAKTERI TERHADAP Streptococcus mutans PENYEBAB PLAK GIGI. Jurnal Imiah Nusantara, 2(3).

Ningsih, D. S., Henri, H., Roanisca, O., & Mahardika, R. G. (2020). Skrining Fitokimia dan Penetapan Kandungan Total Fenolik Ekstrak Daun Tumbuhan Sapu-Sapu (Baeckea frutescens L.). Biotropika: Journal of Tropical Biology, 8(3), 178–185. https://doi.org/10.21776/ub.biotropika.2020.008.03.06

Nuriyah, E., Edi, I. S., & Ulfah, S. F. (2022). Karies Gigi Ditinjau Dari Pengetahuan Kesehatan Gigi Dan Mulut Pada Siswa Sekolah Dasar. Indonesian Journal of Health and Medical, 2(2), 167–179.

Rambe, S., Dalimunthe, G. I., Lubis, M. S., & Yuniarti, R. (2025). Formulasi Sediaan Pasta Gigi Gel Nano Ekstrak Daun Kayu Putih (melaleuca leucadendra l.) sebagai Antibakteri Streptococcus mutans. Jurnal Ilmiah Manusia Dan Kesehatan, 8(1), 265–273. https://doi.org/10.31850/makes.v8i1.3507

Rani, K. C., Imanto, T., Primaharinastiti, R., Hariyadi, D. M., & Hendradi, E. (2019). The Effect of Composite Composition Ratio on the Physicochemical Characteristics of Bovine Hydroxyapatite-Chitosan-Ciprofloxacin Implant. Pakistan Academy of Sciences, 56(4).

Sadiah, H. H., Cahyadi, A. I., & Windria, S. (2022). Kajian Potensi Daun Sirih Hijau (Piper betle L) sebagai Antibakteri. Jurnal Sain Veteriner, 40(2), 128–138.

Sholikhati, A., Dwi Kurnia, S., & Mundriyastutik, Y. (2024). KAJIAN AKTIVITAS FARMAKOLOGIS BUAH PARIJOTO (MEDINILLA SPECIOSA): SISTEMATIK REVIEW. In Jurnal Medika Indonesia (Vol. 5).

Syifaul Qulub, A., Nurdyansyah, F., Muliani Dwi Ujianti, R., Khoiron Ferdiansyah, M., Ayu Widyastuti, D., Rossita Dewi, L., & Rahayu, P. (2022). Penapisan Fitokimia Ekstrak Buah Parijoto (Medinilla Speciosa Blume) Berdasarkan Perbedaan Fraksi. Snse Viii, 1(1), 134–139. Retrieved from http://103.98.176.39/index.php/snse/article/view/3399

Vifta, R. L., & Tinasari, N. D. (2024). Potensi Ekstrak Buah Parijoto (Medinilla speciosa blume) sebagai Bahan Aktif Sedian Antioksidan Facial Wash Gel. Generics: Journal of Research in Pharmacy, 4(1), 86–94. https://doi.org/10.14710/genres.v4i1.23035

Wahidin, Farid, A. M., & Firmansyah. (2021). FORMULASI DAN UJI STABILITAS PASTA GIGI CANGKANG TELUR AYAM RAS (Gallus sp) DENGAN VARIASI KONSENTRASI Na.CMC. Fito Medicine : Journal Pharmacy and Sciences, 12(2), 121–130. Retrieved from http://journal.unpacti.ac.id/index.php/fito

Warida, H., Juliannor, A., & Sukawaty, Y. (2016). Formulasi Pasta Gigi Gel Ekstrak Etanol Bawang Dayak ( Eleutherine bulbosa ( Mill.) Urb.). Jurnal Sains Farmasi & Klinis, 3(1), 42–49.