Formulasi dan Uji Iritasi Sediaan Body Scrub dari Ekstrak Pegagan (Centella Asiatica) dengan Penambahan Scrubber Cangkang Telur Ayam

Niken Maya Suryani; Hasriyani Hasriyani; Bintari Tri Sukoharjanti

doi:10.31004/koloni.v5i2.996

Authors

Niken Maya Suryani Universitas Muhammadiyah Kudus
Hasriyani Hasriyani Universitas Muhammadiyah Kudus
Bintari Tri Sukoharjanti Universitas Muhammadiyah Kudus

DOI:

https://doi.org/10.31004/koloni.v5i2.996

Keywords:

Body scrub, Centella asiatica, Chicken Eggshell Scrub, Irritation Test, Hedonic Test

Abstract

Centella asiatica is known to possess anti-inflammatory, antioxidant, and skin regeneration activities. The use of natural ingredients in cosmetic formulations has gained increasing attention, including the utilization of chicken eggshell waste, which is rich in calcium carbonate, as an environmentally friendly scrubber alternative to microplastics. This study aimed to formulate and evaluate a body scrub preparation containing Centella asiatica extract at concentrations of 1%, 2%, and 3% combined with chicken eggshell powder as a scrubber agent. The evaluation included physical quality tests consisting of organoleptic properties, pH, homogeneity, viscosity, and spreadability, as well as irritation and hedonic tests on panelist. Data analysis was performed using normality tests (Shapiro–Wilk) and homogeneity of variance tests (Levene or Bartlett), followed by One-Way ANOVA and post hoc analysis. The irritation test was conducted using the open patch method for 5 minutes to observe the presence or absence of irritation reactions. Hedonic testing was carried out using questionnaires assessing aroma, color, and texture preferences. The results showed that Centella asiatica extract contained flavonoids, tannins, and saponins. All formulations (F0, F1, F2, and F3) met the physical quality requirements for body scrub preparations, including organoleptic properties, homogeneity, viscosity, and spreadability. The irritation test demonstrated that all formulations were non-irritating and safe for skin application. The hedonic test indicated that the most preferred formulation by the panelists was F1 containing 1% Centella asiatica extract.

References

Aini, R. N., Listyani, T. A., & Raharjo, D. (2023). Perbandingan Kadar Flavonoid Dan Aktivitas Antioksidan Ekstrak Etanol Dan Infusa Daun Rambutan (Nephelium lappaceum L.) Dengan Metode Abts. Jurnal Ilmiah Wahana Pendidikan, Desember, 9(23), 665–680. Https://Doi.Org/10.5281/Zenodo.10353550

Arribas-López, E., Zand, N., Ojo, O., Snowden, M. J., & Kochhar, T. (2022). A Systematic Review Of The Effect Of Centella Asiatica On Wound Healing. International Journal Of Environmental Research And Public Health, 19(6), 3266. Https://Doi.Org/10.3390/Ijerph19063266

Bahari, R., Kasim, K. F., & Ya’acob, W. S. N. W. (2021). Perlis Sunshine Mango Seed Flour Body scrub: Product Development For Physical And Sensory Properties. Iop Conference Series: Earth And Environmental Science, 765(1), 012035. Https://Doi.Org/10.1088/1755-1315/765/1/012035

Chang, T.-M., Wu, C.-C., Huang, H.-C., Wang, S.-S., Chuang, C.-H., Kao, P.-L., Tang, W.-H., Liu, L. T.-C., Qiu, W.-Y., Percec, I., Chen, C., & Kuo, T.-Y. (2025). Evaluation Of Effects On Skin Quality Of A Centella Asiatica Extracellular Vesicle-Based Skin Care Formulation: A 28-Day Facial Skin Quality Study. Https://Doi.Org/10.1101/2025.03.04.25323195

Diniz, L. R. L., Calado, L. L., Duarte, A. B. S., & De Sousa, D. P. (2023). Centella Asiatica And Its Metabolite Asiatic Acid: Wound Healing Effects And Therapeutic Potential. In Metabolites (Vol. 13, Number 2). Mdpi. Https://Doi.Org/10.3390/Metabo13020276

Helal, M. B., Sheta, M. S., & Alghonemy, W. Y. (2024). Comparing The Remineralization Potential Of Undemineralized Dentin Powder Versus Chicken Eggshell Powder On Artificially Induced Initial Enamel Carious Lesions: An In-Vitro Investigation. Bmc Oral Health, 24(1), 1048. Https://Doi.Org/10.1186/S12903-024-04778-6

Isnawati, N., & Pujiastuti, A. (2024). Optimasi Formula Handsanitizer Minyak Bunga Cengkeh (Syzygium Aromaticum): Evaluasi Sifat Fisik Dan Uji Antibakteri Terhadap Staphylococcus Aureus. Jurnal Ilmiah Pamenang, 6(1), 53–59. Https://Doi.Org/10.53599/Jip.V6i1.210

Khairunnisa, S., Hakim, A. R., & Audina, M. (2022). Perbandingan Kadar Flavonoid Total Berdasarkan Perbedaan Konsentrasi Pelarut Etanol Dari Ekstrak Daun Pegagan (Centella Asiatica [L] Urban). Journal Of Pharmaceutical Care And Sciences, 3(1), 121–131. Https://Ejurnal.Unism.Ac.Id/Index.Php/Jpcs

Khuanekkaphan, M., Noysang, C., & Khobjai, W. (2020). Anti-Aging Potential And Phytochemicals Of Centella Asiatica, Nelumbo Nucifera, And Hibiscus Sabdariffa Extracts. Journal Of Advanced Pharmaceutical Technology & Research, 11(4), 174. Https://Doi.Org/10.4103/Japtr.Japtr_79_20

Leny, L., Ginting, I., N Sitohang, T., Fatimah Hanum, S., Hafiz, I., & Iskandar, B. (2021). Formulasi Dan Uji Efektivitas Sediaan Body scrub Labu Kuning (Curcubita Moschata). Majalah Farmasetika, 6(4), 375. Https://Doi.Org/10.24198/Mfarmasetika.V6i4.35776

Nisak, C., Hasriyani, & Sukoharjanti, B. T. (2025). Uji Karakteristik Fisik Formulasi Clay Mask Ekstrak Etanol Daun Ungu (Graptophyllum Pictum L.Griff) Dan Uji Aktivitas Antibakteri Terhadap Staphylococcus Aureus. Nusantara Hasana Journal, 5(2), 221–234. Https://Www.Nusantarahasanajournal.Com/Index.Php/Nhj/Article/View/1534

Nurhidayati, L. G. , Cahyanta, A. N. , & Meilani, A. (2024). Formulasi Dan Uji Stabilitas Fisik Sediaan Lulur Kombinasi Beras Putih (Oryza Sativa.L.) Dan Ampas Kopi Arabika. Jurnal Ilmu Farmasi Dan Kesehatan, 2(1), 24–33.

Ogorzałek, M., Klimaszewska, E., Małysa, A., Czerwonka, D., & Tomasiuk, R. (2024). Research On Waterless Cosmetics In The Form Of Scrub Bars Based On Natural Exfoliants. Applied Sciences, 14(23), 11329. Https://Doi.Org/10.3390/App142311329

Putri, C. E. E., Wulandari, D. M., Hasyim, U. H., Hasyim, D. I., & Ramadhan, M. S. (2024). Optimasi Waktu Maserasi Pada Ekstraksi Daun Pegagan (Centella Asiatica) Terhadap Uji Aktivitas Antioksidan. Prosiding Semnastek, 1–10. Jurnal.Umj.Ac.Id/Index.Php/Semnastek

Rifqi, M. (2021). Ekstraksi Antosianin Pada Bunga Telang (Clitoria Ternatea L.). Sebuah Ulasam. Pasundan Food Technology Journal (Pftj), 8(2), 45–50.

Rizal, R., Salman, & Maharani, V. (2023). Formulasi Sediaan Spray Gel Ekstrak Etanol Pegagan (Centella Asiatica (L.) Urban ) Dan Uji Daya Tabir Surya. Journal Sains Farmasi Dan Kesehatan, 01(01), 48–59. Https://Doi.Org/Https://Doi.Org/10.62379/Jfkes.V1i1.83

Sadik, F., & Rifqah, A. A. A. (2022). Standarisasi Parameter Spesifik Ekstrak Etanol Daun Pegagan (Centella Asiatica L.) Sebagai Antidiabetes. Journal Syifa Sciences And Clinical Research, 4(1), 1–9. Https://Doi.Org/10.37311/Jsscr.V4i1.13310

Sari, M., Chan, A., & Elvani, V. (2023). Formulasi Dan Stabilitas Body scrub Dari Ekstrak Etanol Kulit Manggis (Garcinia Mangostana L.) Sebagai Pelembab Kulit. Ulil Albab: Jurnal Ilmiah Multidisiplin, 2(9), 4314–4320. Https://Repository.Helvetia.Ac.Id/Id/Eprint/5574/1/25.Pdf

Sulistyaningrum, D. E., Rettagung Diana, T., Nurhastuti, R. F., & Prayitno, E. (2024). Inclusive Society Community Servies (Isco) Utilization Of Egg Shell Waste Into A Beauty Facial Mask Inclusive Society Community Servies (Isco). Journal Inclusive Society Community Servies, 2(6), 40–55. Https://Journal.Yayasanpad.Org/Index.Php/Isco/Article/View/203/141

Suryani, M., Susanti, J., & Hutauruk, D. (2024). Formulasi Sediaan Body scrub Ekstrak Etanol Daun Salam (Syzygium Polyanthum (Wight.) Walp) Dan Serbuk Beras Putih (Oryza Sativa L.) Sebagai Pelembab Kulit. Jrikuf: Jurnal Riset Ilmu Kesehatan Umum, 2(4), 137–156. Https://Doi.Org/10.57213/Jrikuf.V2i4.558

Triyanti, S. B., Lestari, F. P., Fitriana, P. A. N., Rostiana, H. R., Silalahi, D. D., Syalsabina, T. D., Putri, R. Y., & Saputra, I. S. (2025). Pengaruh Metode Ekstraksi Maserasi, Sonikasi, Dan Sokletasi Terhadap Nilai Rendemen Sampel Kulit Buah Naga (Hylocereus Polyrhizus). Jurnal Sains Dan Edukasi Sains, 8(1), 71–78. Https://Doi.Org/10.24246/Juses.V8i1p71-78

Yu, G.-R., Kim, D.-H., Kim, H., & Lim, D.-W. (2025). Evaluation Of Saponin-Rich Callus From Saponaria Officinalis L. As A Novel Scrub Material With Significant Exfoliating And Anti-Inflammatory Effects. Plants, 14(10), 1535. Https://Doi.Org/10.3390/Plants14101535

Yuniarsih, N., Siti Nur Rosidah, R., Sukma Ismanita, S., Selvia Putri, N., Mutiah Azzahra, M., Lutfiyah, A., Fitriyani, A., & Sari, K. (2023). Sediaan Bodyscrub Dengan Bahan Alam Sebagai Exfoliating: Literature Riview. Kartika Sari Innovative: Journal Of Social Science Research, 3(2), 7579–7592. Https://J-Innovative.Org/Index.Php/Innovative